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Interferon-gamma release in sympathetically denervated rat submaxillary lymph nodes
P O Castrillón1, D P Cardinali, A Arce
1Departamento de Bioquímica y Biología Molecular III, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
Objective:
To examine the regulation of interferon (IFN)-gamma release by cells derived from submaxillary lymph nodes of rats subjected to an acute or chronic superior cervical ganglionectomy (SCGx).
Methods:
A unilateral SCGx and a contralateral sham operation were performed. Twenty hours or 7 days later cells from submaxillary lymph nodes were incubated for 24 h without any additional treatment (experiment 1), after adding lipopolysaccharide or concanavalin A (experiment 2) or after adding norepinephrine (NE, 10(-8) M; experiment 3). IFN-gamma concentration in the culture media was measured by ELISA.
Results:
Compared to controls, cells obtained from lymph nodes at a time of degeneration of sympathetic nerve terminals released more IFN-gamma, whereas those derived from chronically SCGx lymph nodes released less IFN-gamma. Stimulation of IFN-gamma release by mitogens was detectable in the innervated or acutely denervated lymph nodes, but not in chronically denervated lymph nodes. When the effect of 10(-8) M NE on IFN-gamma release was tested, the neurotransmitter augmented cytokine release in cells prepared from chronically denervated lymph nodes only.
Conclusion:
The microenvironment provided by local sympathetic nerves is essential to enable an appropriate IFN-gamma release by submaxillary lymph node cells to occur.