The disociation of bovine factor V and isolation of the acitivated form

Bovine Factor V disociated non-enzymatically to 276 000 dalton, whereas, the enzymatic activation produced Factor Va of 213 500 daltons. The disociated form, Factor Vd, was not an enzyme substrate for conversion to Factor Va. Factors V and Va sedimented in a density zone of 1.19 g/ml, and Factor Vd in 1.17 g/ml, in sucrose gradient centrifugation. Only a neutral lipid in a 7.2% yield could be extracted from Factor Va which had been isolated in a 4500-fold purification.