Electron transport in an in vitro-reconstituted bacterial photophosphorylating system

Photooxidation of endogenous cytochrome(s) c, photoreduction of endogenous chrome(s) b and photobleaching of bacteriochlorophyll have been demonstrated in an in vitro reconstituted system, previously demonstrated to support photophosphorylation. The kinetic responses of these redox reactions to substrate and antimycin A in these particle are characteristic of electron transport processes and stronglysupport the contention that all, or a part of, the oxidative phosphorylation electron transport pathway can be coupled to reaction center, photopigment complex in a manner which supports photophosphorylation. In addition, a succinate-supported light dependent reduction of NAD+ was found.