Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure and functionality of a designed p53 dimer.

T S Davison1, X Nie, W Ma

  • 1Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Toronto, Ontario, M5G 2M9, Canada.

Journal of Molecular Biology
|March 20, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial DNAs provide insight into trypanosome phylogeny and molecular evolution.

BMC evolutionary biology·2020
Same author

A human huntingtin SNP alters post-translational modification and pathogenic proteolysis of the protein causing Huntington disease.

Scientific reports·2018
Same author

Chemical Biology Approaches for Characterization of Epigenetic Regulators.

Methods in enzymology·2016
Same author

Reducing inequity in primary care clinics treating low socioeconomic Jewish and Arab populations in Israel.

Journal of public health (Oxford, England)·2016
Same author

Authors' reply.

The British journal of psychiatry : the journal of mental science·2014
Same author

Personalized gene silencing therapeutics for Huntington disease.

Clinical genetics·2014
Same journal

Tesorai Search: cloud-based database search engine boosts identifications for mass spectrometry proteomics with a pretrained peptide-spectrum deep-learning model.

Journal of molecular biology·2026
Same journal

Characterization of diverse functions of NRF1 nuclear localization sequence.

Journal of molecular biology·2026
Same journal

UPF3A and UPF3B shape the transcriptome cooperatively yet oppose cell function.

Journal of molecular biology·2026
Same journal

Antibody-secreting cells integrate efficient NMD with non‑canonical UPR signaling to maintain proteostasis and support massive immunoglobulin synthesis.

Journal of molecular biology·2026
Same journal

Small molecule stabilization of diverse amyloidogenic immunoglobulin light chains revealed by hydrogen-deuterium exchange mass spectrometry.

Journal of molecular biology·2026
Same journal

UPF1 at Work: Structural and Mechanistic Insights Into a Master Regulator of Nonsense-Mediated mRNA Decay.

Journal of molecular biology·2026
See all related articles

Altering the p53 protein

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • The tumor suppressor protein p53 is crucial for regulating cell proliferation and death.
  • Oligomerization of p53 is essential for its function.
  • Previous studies altered single residues in the oligomerization domain, affecting solubility and stability.

Purpose of the Study:

  • To investigate the role of p53 oligomerization in its function.
  • To design and characterize novel p53 mutants with altered oligomeric states.

Main Methods:

  • Site-directed mutagenesis to create double mutants at Met340 and Leu344.
  • Nuclear Magnetic Resonance (NMR) spectroscopy for structure determination.
  • In vivo assays to assess cell-cycle arrest and transcriptional transactivation.

Related Experiment Videos

Main Results:

  • Double mutations at Met340 and Leu344 generated distinct dimeric p53 forms.
  • The Met340Gln/Leu344Arg double mutant was structurally characterized as a "half-tetramer".
  • Tetrameric p53 is required for cell-cycle arrest; monomers and dimers show reduced transactivation activity.

Conclusions:

  • p53 oligomerization state critically influences its biological functions.
  • Specific oligomeric forms (tetramers) are essential for tumor suppression.
  • Dimeric and monomeric p53 retain partial transcriptional activity, suggesting distinct roles.