Related Experiment Videos
Generation and characterization of a defined mutant of Streptococcus suis lacking suilysin
A G Allen1, S Bolitho, H Lindsay
1Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom.
Infection and Immunity
|March 20, 2001
Abstract:
A defined allelic-replacement mutant of the sly gene, encoding a thiol-activated cytolysin, from a European isolate of Streptococcus suis serotype 2 was generated and characterized. Unlike the parental strain, it is nonhemolytic, noncytotoxic for cultured macrophage-like cells, avirulent in a mouse infection model, yet only slightly attenuated in a porcine model of systemic infection.