Blood S-100 protein concentration in children undergoing cardiac surgery

To investigate plasma levels of the betabeta isomer of S-100 protein and to assess the relationship between post-cardiopulmonary bypass (CPB) levels of this marker and a variety of perioperative and patient factors in children undergoing cardiac surgery.

Prospective study.

University hospital.

Twenty-five children.

Blood samples (2 mL) for S-100 determinations were collected after the induction of anesthesia, 30 minutes after aortic cross-clamping, 1 hour after the termination of CPB, and 5 and 24 hours after the operation. Electroencephalogram activity was recorded, and neurologic examination was performed on all children 1 day before and 10 days after the operation. Lowest values of nasopharyngeal temperature, mean arterial pressure, arterial carbon dioxide tension (PaCO2), pH, and hematocrit during CPB were recorded.

The overall change in S-100 during the study period was found to be statistically significant (p < 0.0001). Correlation between deltaS-100 and age (r = -0.45; p = 0.04), body surface area (r = -0.63; p = 0.002), nasopharyngeal temperature (r = -0.55; p = 0.01), and PaCO2 (r = -0.55; p = 0.009) was statistically significant in infants and children. Multivariate regression analysis indicated significant effects of PaCO2 and body surface area on deltaS-100 levels and area under the curve values.

In contrast to newborns, infants and older children showed prominent increases in S-100 protein concentration. Lack of pathologic electroencephalogram findings and neurologic signs in the postoperative period precludes the clinical use of S-100 protein concentration as a sensitive marker of cerebral injury.