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Updated: Jul 21, 2026

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Published on: September 11, 2017
Bacterial replication initiator DnaA. Rules for DnaA binding and roles of DnaA in origin unwinding and helicase
W Messer1, F Blaesing, D Jakimowicz
1Max-Planck-Institut für molekulare Genetik, Ihnestrasse 73, 14195, Berlin, Germany. messer@mpimg-berlin-dahlem.mpg.de
Abstract:
We review the processes leading to the structural modifications required for the initiation of replication in Escherichia coli, the conversion of the initial complex to the open complex, loading of helicase, and the assembly of two replication forks. Rules for the binding of DnaA to its binding sites are derived, and the properties of ATP-DnaA are described. We provide new data on cooperative interaction and dimerization of DnaA proteins of E. coli, Streptomyces and Thermus thermophilus, and on the stoichiometry of DnaA-oriC complexes of E. coli.
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