[Detection of 1,4-benzodiazepine derivatives in liver tissues by immunochemical methods]

The possibility of using immunochemical methods in general forensic chemical analysis of cadaveric material for narcotics is demonstrated. Conditions of isolation of 1,4-benzodiasepines from tissues and analysis of the resultant tissue extracts by polarization fluoroimmunoassay (PFIA) and solid-phase enzyme immunoassay are described. Time course of concentrations in tissue extracts stored for a long time has been studied by PFIA. Characteristics of Benzodiazepines Serum kits (Abbott) for PFIA of liver extracts are determined.