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Related Experiment Videos

Analysis of R-Ras signalling pathways.

A J Self1, E Caron, H F Paterson

  • 1MRC Laboratory for Molecular Cell Biology, CRC Oncogene and Signal Transduction Group, University College London, Gower Street, London WC1E 6BT, UK.

Journal of Cell Science
|March 21, 2001
PubMed
Summary

Ras-related protein R-Ras regulates cell growth and adhesion through distinct pathways. Unlike Ras, R-Ras does not activate ERK MAP kinase but influences cell cycle and integrin activation, impacting cellular functions.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Signal Transduction

Background:

  • Ras-related protein R-Ras shares homology with Ras and other Ras subfamily members.
  • Activated Ras and TC21 proteins are transforming, and mutated forms are found in human tumors.
  • R-Ras interacts with proteins like c-Raf1 and can induce transformed foci, though weakly and cell-type specifically.

Purpose of the Study:

  • To investigate R-Ras signaling pathways in various cell types.
  • To elucidate the downstream signaling mechanisms of R-Ras in cellular processes.
  • To differentiate R-Ras signaling from that of Ras.

Main Methods:

  • Microinjection of activated R-Ras into quiescent fibroblasts and PC12 cells.
  • Analysis of MAP kinase pathway activation (ERK, JNK, p38/Mpk2).

Related Experiment Videos

  • Investigation of integrin activation and phagocytosis in a macrophage cell line.

    • Main Results:

    • Microinjected R-Ras stimulated cell cycle progression and DNA synthesis in fibroblasts.
    • R-Ras did not activate ERK, JNK, or p38/Mpk2 MAP kinase pathways.
    • R-Ras induced cell spreading in PC12 cells and activated alpha(M)beta(2) integrin via Rap1 in macrophages, leading to phagocytosis.

    Conclusions:

    • R-Ras plays a significant role in regulating cell growth and adhesion.
    • R-Ras mediates its effects through downstream signaling pathways distinct from those of Ras.
    • These findings highlight R-Ras as a regulator of integrin-mediated processes like phagocytosis.