Related Experiment Video
Updated: Jul 28, 2026
09:41
A Method For Production of Recombinant mCD1d Protein in Insect Cells.
Published on: December 11, 2007
Human CD1d associates with prolyl-4-hydroxylase during its biosynthesis
H S Kim1, S P Colgan, R Pitman
1Division of Gastroenterology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA.
Molecular Immunology
|March 21, 2001
Summary
The study reveals that prolyl-4-hydroxylase (P4H) associates with CD1d during its biosynthesis in intestinal cells. This interaction leads to hydroxyproline modification in the 37-kDa CD1d form, suggesting a novel post-translational modification pathway.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- CD1d proteins present glycolipid antigens to T cells.
- CD1d exists in two forms in human intestinal epithelial cells (IECs): 37-kDa (beta2m independent) and 47-kDa (beta2m dependent).
- The biosynthesis and generation mechanisms of these CD1d forms in IECs are currently unknown.
Purpose of the Study:
- To investigate the biosynthetic pathways and generation mechanisms of distinct CD1d forms in human IECs.
- To identify proteins interacting with CD1d during its biosynthesis.
- To determine if CD1d forms contain hydroxyproline residues.
Main Methods:
- Utilized a human colonic cell line (T84) transfected with CD1d.
- Employed pulse-chase metabolic labeling studies.
- Performed microsequencing and amino acid composition analysis.
Main Results:
- CD1d was found to be a stable protein over a 4-day chase period.
- A 65-kDa glycoprotein, identified as prolyl-4-hydroxylase (P4H) subunits, co-immunoprecipitated with CD1d within 24 hours.
- Amino acid analysis confirmed hydroxyproline residues only in the 37-kDa CD1d form, not the 48-kDa form.
Conclusions:
- CD1d exhibits a prolonged association with P4H during biosynthesis.
- The 37-kDa CD1d form contains hydroxyproline residues, indicating a novel post-translational modification.
- P4H association likely contributes to the generation of distinct CD1d biochemical forms.
Related Concept Videos
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:32Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
01:15Differentiation of Common Myeloid Progenitor Cells
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
01:29Amino Acid Biosynthetic Pathways
Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which provide...