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Nitric oxide synthase type 1 expression in human lung cancer and its relation to p53
B Lewko1, A Zółtowska, J Stepiński
1Department of Immunopathology, Medical University of Gdańsk, ul. Debinki 7, 80-211 Gdansk, Poland. blew@amedec.amg.gda.pl
Background:
The nitric oxide synthases (NOS) have been observed in human tumour cell lines as well as in solid tumours. Neuronal isoform of NOS (NOS1) was particularly abundant in low-differentiated gynaecological, breast and central nerve system tumours, suggesting that it may characterize poorly differentiated tumours. So far, little is known about expression of the neuronal NOS isoform in non-small cell lung cancer. Our aim was to compare NOS1 expression in non-small lung carcinomas with respect to tumor staging and p53 protein expression.
Material And Methods:
Thirty-two cases of non-small lung carcinomas of all grades of malignancy and ten control lung specimens with neoplastic lesions were examined. Paraffin-embedded tissues were stained with hematoxylin and eosin, or with antibodies to NOS1 and p53, and evaluated under light microscope.
Results:
No statistical correlation between expression of p53, NOS1 and degree od tumour differentiation was observed.
Conclusion:
Expression of NOS1 can not serve as a marker for highly malignant tumours in the non-small cell lung carcinomas.