Electrical-to-mechanical coupling in purple membranes: membrane as electrostrictive medium

In this paper, we present acousto-electrical measurements performed on dry films of purple membranes (PM) of Halobacterium salinarium. The purpose of these measurements is to determine the relation between mechanical and electrical phenomena in bacteriorhodopsin and to define the role of the protein in the proton transfer process. Electrical-to-mechanical coupling in PMs manifests itself as direct and inverse piezoelectric effects. Measurements performed on the samples with different degrees of PM orientation and at various values of the externally applied cross-membrane electric field indicate that piezoelectric phenomena in PMs arise from the electric asymmetry of the membranes, i.e., they originate from electrostriction. Experiments with samples made of oriented PMs allow estimation of the value of the intrinsic cross-membrane electric field, which is approximately 10(8) V/m. A hypothetical model of PM is presented where the electrical-to-mechanical coupling is suggested to be the main driving force for the proton translocation against the Coulomb forces acting in the membrane.