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Updated: Jul 16, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 18, 2010
Factors affecting short-term and long-term stabilities of proteins
T Arakawa1, S J Prestrelski, W C Kenney
1Amgen Inc., Amgen Center, Thousand Oaks, CA, USA. tarakawa2@aol.com
Abstract:
Proteins are marginally stable and, hence, are readily denatured by various stresses encountered in solution, or in the frozen or dried states. Various additives are known to minimize damage and enhance the stability of proteins. This review discusses the current knowledge of the mechanisms by which these additives stabilize proteins against acute stresses, and also the various factors to be considered for long-term storage of proteins in solution.
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