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Updated: Aug 13, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
Resistance to activated protein C due to factor V Leiden mutation: high prevalence in patients with post-thrombotic
Y Gaber1, H J Siemens, W Schmeller
1Department of Dermatology, Medical University of Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany.
Background:
Activated protein C (APC) resistance is the most frequently diagnosed heritable thrombophilic defect predisposing to thrombosis.
Objectives:
To determine the prevalence of APC resistance due to factor V Leiden mutation in patients with leg ulcers.
Methods:
Within a 2-year-period 100 consecutive patients with leg ulcers were examined for factor V Leiden mutation.
Results:
APC resistance due to factor V Leiden mutation was detected in 19 of 53 patients (36%) with post-thrombotic leg ulcers and in three of 47 patients (6%) with ulcers caused by primary varicosis. In a healthy control group APC resistance due to factor V Leiden mutation was found in five of 96 (5%) volunteers.
Conclusions:
In view of this high prevalence of APC resistance of 36%, which has never previously been reported, patients with post-thrombotic leg ulcers should be investigated for APC resistance.
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