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Apoptotic activity in gestational trophoblastic disease correlates with clinical outcome: assessment by the

P M Chiu1, Y S Ngan, U S Khoo

  • 1Department of Pathology, The University of Hong Kong, Queen Mary Hospital, Pokfulam Road, Hong Kong.

Histopathology
|March 22, 2001
PubMed
Abstract

Insights

Apoptosis plays a key role in gestational trophoblastic disease (GTD). Measuring apoptotic activity in hydatidiform mole (HM) using the M30 index can predict clinical behavior.

Area of Science:

  • Gynecology
  • Oncology
  • Pathology

Background:

  • Gestational trophoblastic disease (GTD) encompasses a spectrum of placental disorders.
  • Hydatidiform mole (HM) is a common form of GTD with varying clinical outcomes.
  • Understanding the pathobiology of GTD is crucial for accurate diagnosis and prognosis.

Purpose of the Study:

  • To evaluate apoptotic activity in GTD.
  • To determine the prognostic significance of apoptosis in hydatidiform mole (HM).

Main Methods:

  • Immunohistochemistry was used to assess apoptosis via the M30 CytoDeath antibody, targeting caspase cleavage sites in cytokeratin 18.
  • Analyzed samples included normal placentas, spontaneous abortions, partial and complete HM, and choriocarcinoma.
  • Compared M30 index with TUNEL and Ki67 proliferation index.

Main Results:

  • Significantly higher M30 index in HM and choriocarcinoma compared to normal placentas and spontaneous abortions (P < 0.001).
  • HM with spontaneous regression showed a higher M30 index than those requiring chemotherapy (P < 0.001).
  • M30 index correlated with TUNEL and Ki67 indices (P = 0.007 and P = 0.034, respectively).

Conclusions:

  • Apoptosis is integral to the pathogenesis of GTD.
  • The M30 index serves as a valuable prognostic indicator for predicting the clinical course of HM.

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