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Related Experiment Videos

A novel HLA-DRB1 sequence, DRB1*11272.

S Ulrich1, W Helmberg, T Wagner

  • 1Department of Blood Group Serology and Transfusion Medicine, Universitaetsklinik Graz, Graz, Austria.

Tissue Antigens
|March 22, 2001
PubMed
Summary
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Detection of a new HLA-A allele, designated HLA-A*32:53.

Tissue antigens·2013

We identified a new Human Leukocyte Antigen - DRB1 (HLA-DRB1) allele, designated DRB1*11272. This novel allele is distinguished by a synonymous mutation at codon 77, resulting in a new HLA-DRB1 sequence.

Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Human Leukocyte Antigen (HLA) system

Background:

  • The Human Leukocyte Antigen (HLA) complex plays a crucial role in immune response and transplantation.
  • Genetic variations within HLA genes, particularly HLA-DRB1, contribute to immune system diversity and disease susceptibility.

Purpose of the Study:

  • To characterize and report the complete exon 2 sequence of a novel HLA-DRB1 allele.
  • To detail the specific genetic mutation differentiating the new allele from previously identified ones.

Main Methods:

  • DNA sequencing of the HLA-DRB1 gene.
  • Bioinformatic analysis to identify and compare allele sequences.
  • Comparison with existing HLA allele databases and sequence repositories.

Related Experiment Videos

Main Results:

  • The complete exon 2 sequence of the novel HLA-DRB1 allele, designated DRB1*11272, was determined.
  • DRB1*11272 differs from DRB1*11271 by a single synonymous mutation (AAT to AAC) at codon 77, both encoding asparagine.
  • This specific codon 77 motif is shared with other HLA-DRB1 alleles, including DRB1*1107, DRB1*1333, DRB1*0422, and most DRB1*03 alleles.

Conclusions:

  • A new HLA-DRB1 allele, DRB1*11272, has been identified and its exon 2 sequence fully described.
  • The identification of novel HLA alleles is essential for high-resolution HLA typing and understanding immune system variation.
  • This finding contributes to the growing database of HLA polymorphism, important for immunological research and clinical applications.