Related Experiment Video
Updated: Jul 11, 2026

Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
Published on: July 11, 2012
Characterization for didodecyldimethylammonium bromide liquid crystal film entrapping catalase with enhanced direct
1Department of Chemistry, Fudan University, Shanghai, People's Republic of China.
Abstract:
Direct electrochemistry for catalase (CAT) embedded in the liquid crystal film of didodecyldimethylammonium bromide (DDAB) was investigated at pyrolytic graphite (PG) electrode by voltammetric methods. The reduction reaction involved the redox couple in CAT, i.e. FeIII/FeII couple. The electron transfer between the incorporated CAT and PG electrode was found to be greatly enhanced by DDAB. The heterogeneous electron transfer rate constant k(s) was fitted as 3.0 +/- 0.4 s(-1) using the nonlinear regression analysis of the square wave voltammograms at a series of pulse heights. The pH dependence of the formal potential for CAT in DDAB film was 57 mV/pH, which suggested one-proton-transfer together with a one-electron reaction. Visible absorption and reflectance-absorbance infrared (RAIR) spectra inferred the similar heme environment of CAT in DDAB film to its native status. Circular dichroism (CD) results indicated DDAB affected slightly on the second structure of CAT.
Related Concept Videos
Catalysis
Introduction to Mechanisms of Enzyme Catalysis
Catalysis

