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Related Experiment Videos

Problems associated with measuring phytate in infant cereals.

S P Brooks1, B J Lampi

  • 1Nutrition Research Division, Health Protection Branch, Health Canada, PL2203C Banting Research Centre, Tunney's Pasture, Ottawa, ON, Canada K1A 0L2. Steve_Brooks@hc-sc.gc.ca

Journal of Agricultural and Food Chemistry
|March 23, 2001
PubMed
Summary

Ion exchange HPLC is the preferred method for accurately measuring inositol hexaphosphate (IP6) in infant cereals. Ion pair HPLC significantly underestimates IP6 due to mineral interference in these products.

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Area of Science:

  • Analytical Chemistry
  • Food Science
  • Nutritional Biochemistry

Background:

  • Inositol hexaphosphate (IP6), also known as phytate, is a major phosphorus storage compound in plant seeds.
  • Infant cereals are a common food source for infants, and their IP6 content can affect mineral bioavailability.
  • Accurate measurement of IP6 is crucial for assessing the nutritional quality of infant foods.

Purpose of the Study:

  • To compare the accuracy of ion pair HPLC and ion exchange HPLC for measuring IP6 in infant cereals.
  • To identify factors contributing to discrepancies between the two methods.
  • To recommend the most suitable method for IP6 analysis in infant cereals.

Main Methods:

  • Commercially available dried infant cereals were analyzed for IP6 content using both ion pair HPLC and ion exchange HPLC.

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  • Recovery studies were performed by adding known amounts of IP6 to cereal samples.
  • Mineral content (calcium and iron) of the samples was determined.

    • Main Results:

    • Ion pair HPLC yielded IP6 values 14 to 190-fold lower than ion exchange HPLC.
    • Recovery of added IP6 by ion pair HPLC was poor (25-60%).
    • High mineral content (calcium and iron) in infant cereals was found to interfere with IP6 measurement by ion pair HPLC, likely through sequestration.

    Conclusions:

    • Ion exchange HPLC is the method of choice for accurate IP6 determination in infant cereals.
    • The high mineral content in infant cereals can cause significant underestimation of IP6 when using ion pair HPLC.
    • Understanding analytical method limitations is critical for accurate nutritional assessment of infant foods.