Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immune functions in splenectomized thalassaemic children.

J Ahluwalia1, U Datta, R K Marwaha

  • 1Department of Immunopathology and Pediatrics, Postgraduate Institute of Medical Education and Research, Chandigarh.

Indian Journal of Pediatrics
|March 27, 2001
PubMed
Summary

Splenectomy in children with thalassemia major may alter immune function, affecting lymphocyte counts and CD4/CD8 ratios. Further research is needed to clarify the exact mechanisms behind these immune changes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy and safety of various oral regimens (three oral doses) and schedules (daily <i>v</i>. monthly) of cholecalciferol in North Indian adults with low vitamin D status: evidence from a randomised controlled trial.

The British journal of nutrition·2022
Same author

Indian Academy of Pediatrics Revised (2021) Guidelines on Prevention and Treatment of Vitamin D Deficiency and Rickets.

Indian pediatrics·2021
Same author

Inherited Bleeding Disorders in North Indian Children: 14 years' Experience from a Tertiary Care Center.

Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion·2020
Same author

An outbreak of meticillin-resistant Staphylococcus aureus colonization in a neonatal intensive care unit: use of a case-control study to investigate and control it and lessons learnt.

The Journal of hospital infection·2019
Same author

Hemophilia in the developing world: transforming lives through international collaboration.

Blood advances·2018
Same author

Point-prevalence survey of carbapenemase-producing Enterobacteriaceae and vancomycin-resistant enterococci in adult inpatients in a university teaching hospital in the UK.

The Journal of hospital infection·2018

Area of Science:

  • Immunology
  • Pediatrics
  • Hematology

Background:

  • Thalassemia major is a genetic blood disorder requiring lifelong treatment.
  • Splenectomy is sometimes performed in thalassemia patients to manage complications.
  • The impact of splenectomy on immune function in these children requires further investigation.

Purpose of the Study:

  • To prospectively assess immune functions in children with thalassemia major who have undergone splenectomy.
  • To compare immune parameters between splenectomized thalassemic children, non-splenectomized thalassemic children, and healthy controls.

Main Methods:

  • Prospective study involving three groups: 10 splenectomized thalassemic children, 10 non-splenectomized thalassemic children, and 6 age-matched controls.
  • Assessment of serum immunoglobulin (IgG, IgA) concentrations.

Related Experiment Videos

  • Evaluation of polymorphonuclear leukocyte (PMN) function using Nitroblue tetrazolium (NBT) dye reduction.
  • Analysis of phytohemagglutinin-induced lymphocyte proliferation.
  • Measurement of absolute lymphocyte count and CD4/CD8 ratio.

    • Main Results:

    • Serum IgG and IgA levels were higher in splenectomized children, though not statistically significant.
    • Unstimulated NBT dye reduction was significantly higher in non-splenectomized children compared to the other groups.
    • Phytohemagglutinin-induced mitogen proliferation was normal across all groups.
    • Splenectomized children had a significantly higher absolute lymphocyte count and a lower CD4/CD8 ratio.

    Conclusions:

    • Splenectomy appears to alter the immune status of children with thalassemia major.
    • Observed changes include increased absolute lymphocyte counts and a decreased CD4/CD8 ratio.
    • The precise immunological mechanisms underlying these alterations following splenectomy remain unclear.