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Bilobalide promotes expression of glial cell line-derived neurotrophic factor and vascular endothelial growth factor
S X Zheng1, L J Zhou, Z L Chen
1Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China.
Aim:
To study the effects of bilobalide on the expression of glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF) in rat astrocytes in vitro.
Methods:
Semiquantification polymerase chain reaction (SQ-PCR) was used to investigate GDNF and VEGF mRNA expression in the astrocytes after bilobalide (5, 15, 50, 100 mumol.L-1) treatment. Immunohistochemistry method was used to detect GDNF and VEGF protein expression in cells treated with bilobalide 50 mumol.L-1 for 24 h.
Results:
GDNF and VEGF mRNA increased markedly after astrocytes were treated with bilobalide 50 mumol.L-1 for 12 h. GDNF and VEGF protein were detected in the cytoplasm of astrocytes after the cells were treated with bilobalide 50 mumol.L-1 for 24 h.
Conclusion:
Bilobalide induced GDNF and VEGF expression in the cultured astrocytes.
