Related Experiment Video
Updated: Aug 13, 2026
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
Published on: October 1, 2012
[Experimental study on mice scheduled-bone marrow transplantation]
1Institute of Radiation Medicine, Second Military Medical University, Shanghai 200433.
Objective:
To enhance the grafting efficiency of bone marrow transplantation.
Methods:
Lethally irradiated recipient Kunming mice were transplanted with bone marrow cells from normal Kunming mice either in one large (10(7)) number (BMT group) or in four separate small (10(5)-10(6)) numbers (scheduled BMT group) and the survival rate, hematopoiesis reconstitution and acute graft versus host disease (aGVHD) were compared between the two groups.
Results:
Both BMT and SBMT (10(5) x 4) groups obtained the same survival rate of 30%. In SBMT (10(6) x 4) group, the peripheral WBC count, bone marrow nucleated cells and CFU-E, CFU-GM, CFU-S and CFU-F yields all returned to normal 17 days after irradiation. The degree of GVHD in SBMT group was less severe than that in BMT group, and the survival rate (60%) was significantly higher than that in BMT group (30%).
Conclusion:
SBMT can significantly enhance the grafting efficiency.
