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Standardized flow cytometry gating in veterinary medicine

K M Byrne1, G A Reinhart, M G Hayek

  • 1Department of Animal Sciences, Washington State University, P.O. Box 646351, Pullman, WA 99164-6351, USA. kbyrne@wsu.edu

Methods in Cell Science : an Official Journal of the Society for in Vitro Biology
|March 27, 2001
PubMed

Gating in flow cytometry is used to select subpopulations of cells for analysis. The technique is critical for subsequent analysis in order to select the population, free of debris and unrelated cells. Accurately quantifying subpopulations in clinical cases is necessary for correct diagnosis. Human lymphocytes are selected by backgating on populations of CD45+high CD14- cells. These reagents are not available widely across species. In veterinary medicine, markers to identify lymphocytes are usually limited to T-lymphocyte, CD4, CD8, and B-lymphocyte surface antigens. A standardized gating technique using a T-lymphocyte antibody is described and is applicable across species where limited phenotype markers are available.

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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