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Carboproteins: a 4-alpha-helix bundle protein model assembled on a D-galactopyranoside template
1Department of Organic Chemistry, Technical University of Denmark, Lyngby.
Bioorganic & Medicinal Chemistry Letters
|March 27, 2001
Abstract:
We have recently introduced the concept of monosaccharides as templates for de novo design of protein models and described the synthesis of a model 'carbopeptide'. Here, we report the synthesis of a 64 amino acid (AA) 'carboprotein' by chemoselective ligation of a C-terminal hexadecapeptide aldehyde to a tetra-aminooxy functionalized methyl alpha-D-galactopyranoside (D-Galp) template. Biophysical characterizations by CD spectroscopy and NMR amide H-D exchange experiments indicated that the four-stranded carboprotein forms a 4-alpha-helix bundle structure.