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Complete nucleotide sequence of RNA-2 of Olive latent ringspot virus
R Alkowni1, F Grieco, G P Martelli
1Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università degli Studi, Bari, Italy.
Abstract:
The complete nucleotide sequence of Olive latent ringspot virus (OLRSV) RNA-2 was determined. This RNA is 3969 nucleotides in length and contains a single open reading frame of 3448 nt, that encodes a polypeptide of 1146 amino acids, with a calculated Mr of 126,044. OLRSV RNA-2 has a structural organization typical of nepoviruses, with the coat protein (CP) cistron located in the C-terminal and the putative movement protein (MP) in the N-terminal regions of the polyprotein. Computer-assisted comparison of coat proteins of OLRSV and other nepoviruses disclosed relationships that tally with subgrouping based on physicochemical properties.