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Additional rep-encoding DNAs associated with banana bunchy top virus.

C L Horser1, M Karan, R M Harding

  • 1Centre for Molecular Biotechnology, Queensland University of Technology, Brisbane, Australia.

Archives of Virology
|March 27, 2001
PubMed
Summary

Two new DNA components, BBTV-S1 and S2, were identified in banana bunchy top nanovirus (BBTV) isolates. These components encode replication proteins but are not integral to the BBTV genome, varying geographically.

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Area of Science:

  • Plant virology
  • Molecular biology
  • Genomics

Background:

  • Banana bunchy top nanovirus (BBTV) possesses a multicomponent circular single-stranded DNA (cssDNA) genome.
  • At least six components are known to constitute the BBTV genome.

Purpose of the Study:

  • To clone, sequence, and analyze two additional cssDNA components (BBTV-S1 and S2) from a Taiwanese BBTV isolate.
  • To investigate the genomic organization and phylogenetic relationship of BBTV-S1 and S2 with other BBTV components.
  • To determine the geographical distribution of BBTV-S1 and S2 in different BBTV isolates.

Main Methods:

  • DNA cloning and sequencing of BBTV components.
  • Sequence analysis and comparison of BBTV-S1 and S2 with BBTV DNA-1.
  • Phylogenetic analysis of replication initiation protein (Rep) genes.

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  • Detection of BBTV-S1/S2 in various geographical isolates using molecular methods.

    • Main Results:

    • Two novel cssDNA components, BBTV-S1 (1109 nt) and S2 (1095 nt), were identified and sequenced.
    • BBTV-S1 and S2 potentially encode replication initiation proteins (Reps) but differ in genome organization from BBTV DNA-1.
    • Phylogenetic analysis confirmed BBTV-S1 and S2 are distinct from BBTV DNA-1.
    • BBTV-S1 and S2 were detected in isolates from Vietnam, Taiwan, the Philippines, Tonga, and Samoa, but not in Australian, Egyptian, Fijian, or Indian isolates.

    Conclusions:

    • BBTV-S1 and S2 are not integral components of the BBTV genome.
    • These components represent additional Rep-encoding DNAs associated with BBTV, with a specific geographical distribution.
    • The findings contribute to understanding the genetic diversity and complexity of the BBTV genome.