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11:05
Analysis of Oxidative Stress in Zebrafish Embryos
Published on: July 7, 2014
Antioxidants reduce oxidative stress in claudicants
M H Wijnen1, S A Coolen, H L Vader
1Department of Surgery, Sint Joseph Hospital, Veldhoven, 5500 MB, The Netherlands.
The Journal of Surgical Research
|March 27, 2001
Summary
Antioxidant vitamins E and C supplementation reduced oxidative stress in patients with claudication. This intervention may mitigate ischemia-reperfusion injury and lower cardiovascular risk in this population.
Area of Science:
- Biomedical science
- Cardiovascular research
- Oxidative stress studies
Background:
- Ischemia-reperfusion injury in claudicants causes local and remote tissue damage.
- Oxygen-derived free radicals (ODFR) contribute significantly to this injury.
- Targeting ODFR presents a potential therapeutic strategy.
Purpose of the Study:
- To investigate the effect of vitamin E and C supplementation on oxidative stress in claudicants.
- To assess whether antioxidant administration can reduce markers of oxidative stress during ischemia-reperfusion.
Main Methods:
- Employed a novel method measuring ortho-APOH, a marker of oxidative stress.
- 16 claudicants received daily vitamin E (200 mg) and vitamin C (500 mg) for 4 weeks.
- Oxidative stress was quantified via ortho-APOH levels during a standard walking test before and after supplementation.
Main Results:
- Ortho-APOH levels significantly increased during reperfusion before vitamin administration (P=0.026).
- Following 4 weeks of vitamin supplementation, the reperfusion-induced rise in ortho-APOH was abolished.
- Malondialdehyde levels remained unchanged, indicating specific effects on the measured oxidative stress pathway.
Conclusions:
- Supplementation with antioxidants (vitamins E and C) effectively reduces oxidative stress in claudicants.
- This reduction in oxidative stress may have implications for mitigating remote ischemia-reperfusion damage.
- Potential benefits include a decrease in cardiovascular morbidity for patients with claudication.
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