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Related Experiment Videos

Glutamine utilization in activated lymphocytes from rats receiving endotoxin.

C T Liu1, K M Chen, P L Chang

  • 1Department of Nutrition, Chung Shan Medical College, Taichung, 402, Republic of China. ctl@mercury.csmc.edu.tw

The Journal of Surgical Research
|March 27, 2001
PubMed
Summary

Endotoxin-induced metabolic stress in rats reduces lymphocyte glutamine utilization and proliferation. This decrease in glutamine use by lymphocytes is linked to lower glutaminase activity, impacting immune cell function.

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Area of Science:

  • Immunology
  • Metabolic Biochemistry

Background:

  • Supplemental glutamine may benefit lymphocyte function during metabolic stress.
  • The precise mechanisms of glutamine utilization by stressed lymphocytes remain unclear.
  • This study investigates glutamine use and proliferation in lymphocytes under endotoxin-induced stress.

Purpose of the Study:

  • To determine the effect of endotoxin-induced stress on in vitro glutamine utilization by lymphocytes.
  • To assess the impact of stress on glutamine-dependent proliferation of activated lymphocytes.
  • To explore the relationship between glutamine metabolism and lymphocyte function post-stress.

Main Methods:

  • Metabolic stress was induced in rats via intraperitoneal endotoxin injection.
  • Lymphocytes were isolated at various time points (6, 12, 24, 48 hours) post-injection.

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  • Lymphocyte proliferation, glutamine utilization rate, and glutaminase activity were measured in vitro.

    • Main Results:

    • Lymphocyte proliferation was suppressed 12, 24, and 48 hours after endotoxin administration.
    • The glutamine concentration required for maximal lymphocyte response decreased over time post-stress.
    • Both glutamine utilization rate and glutaminase activity in activated lymphocytes showed a time-dependent decrease.

    Conclusions:

    • Glutamine utilization by lymphocytes is significantly reduced in the late phase after endotoxin injection.
    • Decreased glutaminase activity contributes to the reduced glutamine utilization.
    • Impaired glutamine metabolism is associated with suppressed proliferation of mitogen-activated lymphocytes.