Related Experiment Video
Updated: Aug 11, 2026

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo
Published on: November 8, 2013
MPtopo: A database of membrane protein topology
S Jayasinghe1, K Hristova, S H White
1Department of Physiology and Biophysics, University of California, Irvine, California 92697-4560, USA.
Abstract:
The reliability of the transmembrane (TM) sequence assignments for membrane proteins (MPs) in standard sequence databases is uncertain because the vast majority are based on hydropathy plots. A database of MPs with dependable assignments is necessary for developing new computational tools for the prediction of MP structure. We have therefore created MPtopo, a database of MPs whose topologies have been verified experimentally by means of crystallography, gene fusion, and other methods. Tests using MPtopo strongly validated four existing MP topology-prediction algorithms. MPtopo is freely available over the internet and can be queried by means of an SQL-based search engine.
Related Concept Videos
Introduction to Membrane Proteins
Membrane Proteins
Single-pass Transmembrane Proteins
Insertion of Single-pass Transmembrane Proteins in the RER
Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...
Structure of Porins
Membrane Proteins

