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Genetic differences between Escherichia coli O26 strains isolated in Brazil and in other countries
J C Peixoto1, S Y Bando, J A Ordoñez
1Departamento de Immunologia, Instituto de Ciências Biomédicus da Universidade São Paolo, SP, Brazil.
Abstract:
Genomic diversity among 34 strains of Escherichia coli belonging to different serotypes of the O26 serogroup -- encompassing strains from different geographical origins and Shiga toxin-negative Brazilian strains -- was evaluated through random amplified polymorphic DNA (RAPD) analysis. Our results indicate that Brazilian and non-Brazilian O26 strains fall under distinct but closely related differentiation clusters. RFLP-PCR analysis of the fliC gene sequence was done in order to identify the H(-) serotypes and served to confirm the clustering pattern obtained in the dendrogram generated from RAPD data. The epidemiological significance of these data is discussed.