Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ventilatory response to drug-induced hypermetabolism.

S Levine, W E Huckabee

    Journal of Applied Physiology
    |May 1, 1975
    PubMed
    Summary

    Uncouplers of oxidative phosphorylation, like DNP and EMB, increase ventilation. This study shows the primary stimulus arises from tissues outside the brain and carotid bodies, transmitted via the spinal cord.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The risk of stroke after thrombolytic therapy.

    The New England journal of medicine·1992
    Same author

    Effects of repeated maternal separations on the adrenocortical response to stress of preweanling rats.

    Physiology & behavior·1992
    Same author

    Respiratory muscle function and dyspnea in patients with chronic congestive heart failure.

    Circulation·1992
    Same author

    Lung transplantation in a patient with a prior bone marrow transplant.

    Chest·1992
    Same author

    Effect of negative pressure ventilation on ventilatory muscle endurance in patients with severe chronic obstructive pulmonary disease.

    The American review of respiratory disease·1992
    Same author

    Durability of the reproductive axis in eumenorrheic women during 1 yr of endurance training.

    Journal of applied physiology (Bethesda, Md. : 1985)·1992

    Area of Science:

    • Physiology
    • Respiratory Regulation
    • Cellular Metabolism

    Background:

    • Uncouplers of oxidative phosphorylation increase metabolic rate.
    • Increased metabolic rate is known to increase ventilation.
    • The precise mechanism driving this ventilatory increase remains unclear.

    Purpose of the Study:

    • To investigate the origin of the ventilatory stimulation caused by uncouplers of oxidative phosphorylation.
    • To differentiate the roles of arterial chemoreceptors, central respiratory centers, and peripheral tissues in mediating hypermetabolism-induced ventilation.

    Main Methods:

    • Infusion of 2,4-dinitrophenol (DNP) or ethyl methylene blue (EMB) in anesthetized dogs.
    • Selective head perfusion experiments to isolate the effects of substances administered to the head versus the body.
    • Measurement of oxygen consumption (VO2) and minute ventilation (VE).
    • Assessment of ventilatory responses to hypoxia and hypercapnia in head-perfused preparations.

    Main Results:

    • DNP and EMB infusions significantly increased VO2 and VE.
    • In vagotomized dogs with isolated head perfusion, systemic DNP/EMB administration still increased VE.
    • Selective administration of DNP/EMB to the head resulted in a markedly smaller increase in VE compared to systemic administration.
    • Ventilatory responses to low oxygen and high CO2 were attenuated in head-perfused animals.

    Conclusions:

    • A significant portion of the ventilatory stimulation from DNP/EMB originates in peripheral tissues, not solely from arterial chemoreceptors or the brain.
    • Afferent pathways in the cervical spinal cord likely transmit this peripheral stimulus to the respiratory control center.

    Related Experiment Videos