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Advances in sample preparation in electromigration, chromatographic and mass spectrometric separation methods
M Gilar1, E S Bouvier, B J Compton
1Waters Corp., Milford, MA 01757, USA. martin_gilar@waters.com
Journal of Chromatography. A
|March 28, 2001
Summary
This review covers advanced sample preparation techniques for analyzing DNA, proteins, and drugs in complex biological samples. It highlights methods like solid-phase extraction (SPE) for improved analytical success.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Pharmaceutical Analysis
Background:
- Sample preparation quality is crucial for successful chemical analysis.
- Historically focused on pharmaceutical compounds in biological matrices.
- Current challenges involve complex biochemical samples for genotypic/phenotypic information (DNA, proteins).
Purpose of the Study:
- To review sample pretreatment methods for biopolymers and small drugs in complex matrices.
- To discuss advancements in sample clean-up techniques.
- To meet the evolving demands of biochemical sample analysis.
Main Methods:
- Solid-phase extraction (SPE) sorbent development.
- On-line SPE techniques.
- Membrane-based sample preparation.
- Biopolymer clean-up for mass spectrometry.
Main Results:
- Advances in SPE sorbents enhance extraction efficiency.
- On-line SPE and membrane techniques offer streamlined workflows.
- Effective biopolymer clean-up is essential for mass spectrometry.
Conclusions:
- Modern sample preparation is vital for analyzing complex biological molecules and drugs.
- Innovations in SPE and membrane technologies improve analytical outcomes.
- This review provides insights into current and future sample preparation strategies.