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Antigens of Fusarium solani: types and criteria for immunochemical characterization
O López Jodra1, J M Torres-Rodríguez, A Martínez Gárate
1Grup de Recerca en Micologia Experimental i Clinica, Universitat Autónoma, Barcelona, Spain. olopez@imim.es
Background:
There are no established methods for the preparation and standardization of Fusarium solani antigens. This lack of standardization makes it difficult to use these antigens in allergenic diagnostic tests.
Objective:
To obtain an appropriate standardized method for the preparation of the different antigen types of F. solani.
Methods:
Production of fungal extracts, followed by biochemical and immunological characterization.
Results:
The somatic antigens presented the greatest protein content most of these proteins are common to the metabolic and hydrosoluble antigens, particularly those proteins at 35-39 kDa, 29-32 kDa and 15-16 kDa, as detected by electrophoresis and immunoblotting. The hydrosoluble antigens presented the highest protein diversity; these proteins were the most specific, showing minor determinants in common with the other antigens.
Conclusions:
It is recommended that a mixture of the different antigen sources be used in order to obtain extracts which would cover the maximum number of diagnostic possibilities.
