Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differences between predicted and observed sequences in Saccharomyces cerevisiae.

A Santucci1, L Trabalzini, L Bovalini

  • 1Dipartimento di Biologia Molecolare, Università degli Studi di Siena, Italy. santucci@unisi.it

Electrophoresis
|March 29, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The International Guideline for the Definition, Classification, Diagnosis and Management of Urticaria.

Allergy·2026
Same author

Early onset absence epilepsy of childhood: Epidemiologic data, treatment and outcome in a sample of 56 patients born between 2000 and 2018.

Seizure·2024
Same author

Descriptive analyses of maternally-derived antibody levels against porcine circovirus 2 (PCV-2) in 3- and 21-day-old piglets from farms of four European countries using different vaccination protocols in sows.

Porcine health management·2022
Same author

Risk assessment procedures in Argentina for the safe import, quarantine and release of biological control agents against pests.

Revue scientifique et technique (International Office of Epizootics)·2022
Same author

Corrigendum to "Non-indigenous macrozoobenthic species on hard substrata of selected harbours in the Adriatic Sea" [Mar. Pollut. Bull. 147 (2019); 150-158].

Marine pollution bulletin·2021
Same author

Injury narratives in occupational safety and health prevention in Italy.

Occupational medicine (Oxford, England)·2019
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

This study analyzed wine yeast protein composition, revealing differences between DNA-coded and actual protein structures. Proteomics is crucial for understanding the genome-proteome correlation.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Yeast Genetics

Background:

  • Understanding protein variations in Saccharomyces cerevisiae is vital for enology.
  • Genomic data alone may not fully represent cellular protein profiles.

Purpose of the Study:

  • To investigate the protein composition of a specific Saccharomyces cerevisiae wine yeast strain (K310).
  • To compare experimentally determined protein characteristics with genomic database information.
  • To explore discrepancies between genotypic and phenotypic protein sequences.

Main Methods:

  • Two-dimensional gel electrophoresis to resolve approximately 2,500 protein spots.
  • Determination of experimental molecular masses and isoelectric points.
  • Edman microsequencing for N-terminal and internal protein sequence analysis.

Related Experiment Videos

Main Results:

  • Characterization of protein spots with observed molecular masses ranging from 8-250 kDa.
  • Successful sequencing of N-terminal regions for 12 out of 27 proteins.
  • Identification of differences between experimentally derived protein sequences and SWISS-PROT database entries.

Conclusions:

  • Observed sequence variations indicate post-translational modifications or other changes to proteins.
  • Detailed proteomics is essential to complement genomic sequencing for accurate biological insights.
  • Establishing a precise correlation between an organism's genome and its proteome requires integrated approaches.