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Human ERp29: isolation, primary structural characterisation and two-dimensional gel mapping.

M J Hubbard1, N J McHugh

  • 1Department of Biochemistry, University of Otago, Dunedin, New Zealand. mike.hubbard@stonebow.otago.ac.nz

Electrophoresis
|March 29, 2001
PubMed
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Researchers identified a single human gene encoding ERp29, a widely expressed endoplasmic reticulum protein. This discovery unifies previously identified ERp29 homologues and establishes it as a new protein class, aiding future research into its function.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genomics

Background:

  • A novel 29 kDa endoplasmic reticulum protein (ERp29) was previously characterized in rats.
  • Several ERp29-like gene products exist in human tissues, but their relationships are unclear.

Purpose of the Study:

  • To clarify the relationships between human ERp29-like proteins and rat ERp29.
  • To characterize human ERp29 and its genetic origins.

Main Methods:

  • Isolation and characterization of ERp29 from human liver.
  • Primary structural analysis and two-dimensional gel mapping.
  • Comparison with rat ERp29 and analysis of human genome and proteome data.

Main Results:

  • Human ERp29 shows striking homologies to rat ERp29 in sequence and physical properties.

Related Experiment Videos

  • Two previously reported human homologues (UL35 and ERp31) were identified as ERp29.
  • Human ERp29 is encoded by a single gene on chromosome 12, universally expressed across tissues.

    • Conclusions:

    • Various ERp29 homologues are products of a single gene orthologous to rat ERp29.
    • ERp29 is the sole known member of a new protein class.
    • Established links between genome, proteome, and murine models will facilitate ERp29 function studies in health and disease.