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Production, purification and properties of microbial phytases
A Pandey1, G Szakacs, C R Soccol
1Biotechnology Division, Council of Scientific and Industrial Research, Trivandrum, India. pandey@csrrltrd.ren.nic.in
Bioresource Technology
|March 29, 2001
Summary
Microbial phytases, enzymes that break down phytate, offer a solution to environmental phosphorus pollution. This review covers their production, purification, and properties for commercial use.
Area of Science:
- Biochemistry
- Environmental Science
- Microbiology
Background:
- Phytate is the primary storage form of phosphorus in plants.
- High-phosphate manure leads to environmental pollution.
- Phytases can mitigate phosphorus pollution by releasing phosphate from phytate.
Purpose of the Study:
- To review recent advancements in microbial phytase production.
- To discuss purification techniques for microbial phytases.
- To summarize the properties of microbial phytases.
Main Methods:
- Review of scientific literature on microbial phytase.
- Analysis of production methods from microbial sources.
- Examination of purification strategies and characterization of phytase properties.
Main Results:
- Microbial phytases, particularly from Aspergillus species, are commercially viable.
- Phytases exhibit broad substrate specificity with optimal activity at specific pH and temperature ranges.
- Immobilization enhances phytase thermostability.
Conclusions:
- Microbial phytases are valuable tools for controlling phosphorus pollution.
- Further research into production and purification can optimize commercial applications.
- Understanding phytase properties is crucial for effective industrial use.