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Updated: Oct 9, 2026

Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission
Published on: April 22, 2021
Three-dimensional localisation of fluorescence resonance energy transfer in living cells under two-photon excitation
M G Xu1, B Crimeen, M J Ludford-Menting
1Centre for Micro-Photonics, School of Biophysical Sciences and Electrical Engineering, Swinburne University of Technology, Hawthorn, Victoria, Australia.
Abstract:
Three-dimensional (3-D) imaging of fluorescence resonance energy transfer (FRET) in human cells under two-photon excitation was demonstrated in this study. A sample was prepared by expressing a donor and an acceptor in living cells and using an antibody to secure the proximity of contact between the donor and the acceptor. The quenching of fluorescence emission of a donor in the double-labelled cells indicates the presence of FRET that occurred in these living cells. Because of the quadratic relation of the excitation power, 3-D localisation of FRET becomes possible.
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