Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Actin assembly at membranes controlled by ARF6.

D A Schafer1, C D'Souza-Schorey, J A Cooper

  • 1Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA.

Traffic (Copenhagen, Denmark)
|March 29, 2001
PubMed
Summary

ADP-ribosylation factor-6 (ARF6) regulates actin assembly, driving vesicle movement and fluid uptake in cells. This GTPase controls actin remodeling at cell surfaces, impacting cellular processes like pinocytosis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neurodegeneration in Niemann-Pick Type C disease and Huntington's disease: impact of defects in membrane trafficking.

Current drug targets·2009
Same author

Efficient uptake of Yersinia pseudotuberculosis via integrin receptors involves a Rac1-Arp 2/3 pathway that bypasses N-WASP function.

Molecular microbiology·2001
Same author

An essential role for ARF6-regulated membrane traffic in adherens junction turnover and epithelial cell migration.

The EMBO journal·2001
Same author

Actin dynamics: assembly and disassembly of actin networks.

Current biology : CB·2001
Same author

Cortactin localization to sites of actin assembly in lamellipodia requires interactions with F-actin and the Arp2/3 complex.

The Journal of cell biology·2000
Same author

ADP-ribosylation factor 6 regulates actin cytoskeleton remodeling in coordination with Rac1 and RhoA.

Molecular and cellular biology·2000

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • ADP-ribosylation factor-6 (ARF6) is a small GTPase involved in membrane traffic and actin cytoskeleton remodeling.
  • Understanding ARF6's precise role in actin assembly is crucial for deciphering its cellular functions.

Purpose of the Study:

  • To investigate the function and mechanism of ARF6 in controlling actin assembly.
  • To elucidate the downstream effectors and signaling pathways regulated by ARF6.

Main Methods:

  • Real-time video analysis of actin assembly in living cells.
  • Expression of activated ARF6 to mimic its GTP-bound state.
  • Analysis of vesicle content and motility.

Main Results:

Related Experiment Videos

  • Activated ARF6 induced actin assembly, leading to the motility of vesicle-like particles, including pinosomes.
  • ARF6 stimulated actin assembly at ventral cell surface foci and enhanced fluid phase pinocytosis.
  • ARF6-induced particle motility required Arp2/3 complex, tyrosine kinase activity, phospholipase D (PLD), and D3-phosphoinositides, but not PI(4,5)P2.

Conclusions:

  • ARF6 plays a key role in regulating actin assembly for the motility of pinosomes.
  • ARF6 controls actin dynamics at specific cellular foci on the ventral cell surface.
  • ARF6 integrates signals through PLD and D3-phosphoinositides to modulate actin assembly and membrane trafficking.