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Updated: Jul 29, 2026
High-throughput Yeast Plasmid Overexpression Screen
Published on: July 27, 2011
A large-scale overexpression screen in Saccharomyces cerevisiae identifies previously uncharacterized cell cycle
L F Stevenson1, B K Kennedy, E Harlow
1Massachusetts General Hospital Cancer Center, Building 149, 13th Street, Charlestown, MA 02129, USA. stevenson@helix.mgh.harvard.edu
Abstract:
We have undertaken an extensive screen to identify Saccharomyces cerevisiae genes whose products are involved in cell cycle progression. We report the identification of 113 genes, including 19 hypothetical ORFs, which confer arrest or delay in specific compartments of the cell cycle when overexpressed. The collection of genes identified by this screen overlaps with those identified in loss-of-function cdc screens but also includes genes whose products have not previously been implicated in cell cycle control. Through analysis of strains lacking these hypothetical ORFs, we have identified a variety of new CDC and checkpoint genes.
