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Related Experiment Videos

Tip60 is a cell-type-specific transcriptional regulator.

F Hlubek1, C Löhberg, J Meiler

  • 1Department of Pathology, University of Erlangen-Nürnberg, Krankenhausstr. 8-10, 91054 Erlangen, Germany.

Journal of Biochemistry
|March 29, 2001
PubMed
Summary

Tip60 protein regulates transcription differently in various cell types, impacting HIV-1 Tat activity. Its histone acetyltransferase (HAT) domain is not essential for this regulation, suggesting cofactor recruitment.

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Virology

Background:

  • Tip60, a histone acetyltransferase (HAT), interacts with HIV-Tat and influences transcription.
  • Previous studies indicate Tip60's role in augmenting Tat-dependent transcription.
  • Cellular context may affect Tip60's function in transcriptional regulation.

Purpose of the Study:

  • To investigate the cell-type-specific function of Tip60 in transcription regulation.
  • To elucidate the role of Tip60's HAT domain in modulating HIV-1 Tat activity.
  • To identify potential interactions of Tip60 with other transcriptional factors.

Main Methods:

  • Comparison of Tip60 activity in HeLa and Jurkat T lymphoma cells.
  • Isolation and characterization of Tip60 variants (Tip60Delta1, Tip60Delta2) from Jurkat cells.

Related Experiment Videos

  • Yeast two-hybrid system to assess Tip60 interaction with ZEB (zinc finger E box binding protein).

    • Main Results:

    • Tip60 augmented HIV-1 Tat activity in HeLa cells but inhibited it in Jurkat cells.
    • Tip60Delta2 variant, lacking the HAT domain, modulated HIV-1 Tat activity similarly to full-length Tip60.
    • Tip60 and ZEB interact and cooperatively inhibit CD4 enhancer/promoter activity in Jurkat cells.

    Conclusions:

    • Tip60 acts as a cell-type-specific transcriptional regulator.
    • The HAT domain of Tip60 is dispensable for both transcriptional activation and inhibition.
    • Tip60 may exert its regulatory function by recruiting distinct cell-type-specific cofactors.