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A rapid and highly sensitive method for diagnosis of equine influenza by antigen detection using immuno-PCR
1Department of Disease Control, Hokkaido University Graduate School of Veterinary Medicine, Sapporo 060-0818, Japan.
The Japanese Journal of Veterinary Research
|March 30, 2001
Abstract:
A rapid and highly sensitive method for diagnosis of influenza by detecting viral antigens using immuno-PCR has been developed. The sensitivity of the immuno-PCR for the detection of the nonstructural protein 1 (NS 1) antigenically common among influenza A viruses was 10(1.0-2.0) times higher than that of RT-PCR for the detection of the viral genome. For the detection of the hemagglutinin (HA) subtype-specific antigen, this assay using anti-HA monoclonal antibodies attained a sensitivity of up to 10(7.0-8.0) times higher than those by virus isolation or by RT-PCR.