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A bacterial artificial chromosome library of Lotus japonicus constructed in an Agrobacterium
A E Men1, K Meksem, M A Kassem
1Department of Botany, University of Queensland, Brisbane, Australia. a.men@botany.uq.edu.au
Molecular Plant-Microbe Interactions : MPMI
|March 30, 2001
Abstract:
We constructed a BAC library of the model legume Lotus japonicus with a 6-to 7-fold genome coverage. We used vector PCLD04541, which allows direct plant transformation by BACs. The average insert size is 94 kb. Clones were stable in Escherichia coli and Agrobacterium tumefaciens.