Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dopamine D2 receptor dimer formation: evidence from ligand binding.

D Armstrong1, P G Strange

  • 1School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, United Kingdom.

The Journal of Biological Chemistry
|March 30, 2001
PubMed
Summary

Dopamine D2 receptors bind radioligands differently based on sodium presence. Raclopride shows negative cooperativity in dimerized receptors without sodium, affecting binding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mycobacterium haemophilum infections in bone marrow transplant recipients.

Transplantation·1995
Same author

Patients' views of priority setting in health care: an interview survey in one practice.

BMJ (Clinical research ed.)·1995
Same author

Direct observations of surgical wound infections at a comprehensive cancer center.

Archives of surgery (Chicago, Ill. : 1960)·1995
Same author

Treatment of opportunistic infections in AIDS.

Lancet (London, England)·1995
Same author

A corporate needs assessment for the purchase of district nursing: a qualitative approach.

Public health·1995
Same author

Crouzon and Apert syndromes: intracranial volume measurements before and after cranio-orbital reshaping in childhood.

Plastic and reconstructive surgery·1995

Area of Science:

  • Neuroscience
  • Pharmacology
  • Molecular Biology

Background:

  • Dopamine D2 receptors are crucial in neurological functions.
  • Radioligand binding assays are key to understanding receptor pharmacology.
  • The influence of ions on receptor-ligand interactions requires detailed study.

Purpose of the Study:

  • To investigate the binding characteristics of [(3)H]spiperone and [(3)H]raclopride to D2 dopamine receptors.
  • To elucidate the role of sodium ions in modulating radioligand binding to D2 receptors.
  • To explore the potential for receptor dimerization and cooperativity in D2 dopamine receptor binding.

Main Methods:

  • Radioligand binding assays using [(3)H]spiperone and [(3)H]raclopride.
  • Saturation binding experiments were performed in the presence and absence of sodium ions.

Related Experiment Videos

  • Competition binding experiments were conducted to assess ligand interactions.

    • Main Results:

    • In the presence of sodium, both radioligands labeled a similar number of D2 receptor sites.
    • Absence of sodium ions reduced [(3)H]raclopride labeling by half compared to [(3)H]spiperone.
    • Raclopride demonstrated noncompetitive inhibition of [(3)H]spiperone binding without sodium, suggesting negative cooperativity.

    Conclusions:

    • Dopamine D2 receptors may exist as dimers.
    • Sodium ions significantly influence the binding kinetics and stoichiometry of radioligands.
    • Raclopride exhibits negative cooperativity in the absence of sodium, impacting both its own binding and that of spiperone across the receptor dimer.