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Protein kinase C-associated kinase (PKK), a novel membrane-associated, ankyrin repeat-containing protein kinase

L Chen1, K Haider, M Ponda

  • 1Massachusetts General Hospital Cancer Center and Harvard Medical School, Charlestown, Massachusetts 02129, USA.

The Journal of Biological Chemistry
|March 30, 2001
PubMed

Insights

A novel protein kinase C-associated kinase (PKK) was identified, showing regulated expression in mouse embryos, suggesting a role in early development. Its association with protein kinase Cbeta (PKCbeta) and membrane localization were characterized.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Developmental Biology

Background:

  • Protein kinase Cbeta (PKCbeta) is a key signaling molecule.
  • The discovery of novel kinases associated with PKC signaling pathways is crucial for understanding cellular regulation.

Purpose of the Study:

  • To clone and characterize a novel kinase associated with PKCbeta.
  • To investigate the expression, localization, and enzymatic activity of the novel kinase, named PKK.
  • To explore the relationship between PKK and PKCbeta.

Main Methods:

  • Cloning of the murine PKK gene based on physical association with PKCbeta.
  • Analysis of PKK expression in mouse embryos.
  • Biochemical assays to determine kinase activity and phosphorylation states.
  • Subcellular localization studies (membrane association).

Main Results:

  • A novel membrane-associated protein kinase, PKK, was cloned and characterized.
  • PKK exhibits regulated expression in mouse embryos, suggesting a role in embryogenesis.
  • PKK possesses kinase activity, associates with membranes, and exists in multiple phosphorylation states.
  • PKK associates with PKCbeta but does not phosphorylate it; PKK may be phosphorylated by PKCbeta.

Conclusions:

  • PKK is a developmentally regulated protein kinase with membrane-associating properties.
  • The physical association between PKK and PKCbeta suggests a potential functional link, though its significance requires further investigation.
  • The human homolog of PKK is located on chromosome 21q22.3.

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