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Tumor suppressor p53 protein is a new target for the metastasis-associated Mts1/S100A4 protein: functional

M Grigorian1, S Andresen, E Tulchinsky

  • 1Department of Molecular Cancer Biology, Institute of Cancer Biology, Danish Cancer Society, Strandboulevarden 49, DK-2100 Copenhagen Ø, Denmark. mg@cancer.dk

The Journal of Biological Chemistry
|March 30, 2001
PubMed

Insights

The calcium-binding protein Mts1 (S100A4) physically interacts with and inhibits the tumor suppressor p53 protein. This interaction disrupts p53

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • The tumor suppressor p53 protein plays a critical role in preventing cancer.
  • Mts1 (S100A4) is a calcium-binding protein implicated in tumor progression.
  • The interaction between Mts1 and p53 has not been previously established.

Purpose of the Study:

  • To investigate the physical and functional interaction between Mts1 and p53.
  • To elucidate the mechanism by which Mts1 affects p53 activity.
  • To determine the role of the Mts1-p53 interaction in tumor development.

Main Methods:

  • Co-immunoprecipitation
  • Affinity chromatography
  • Far Western blot analysis
  • In vitro kinase assays
  • Reporter gene assays
  • Analysis of p53 target gene transcription

Main Results:

  • Mts1 directly binds to the C-terminal regulatory domain of p53.
  • Mts1 inhibits p53 phosphorylation by protein kinase C.
  • Mts1 binding impairs p53 DNA binding and transactivation activity.
  • Mts1 differentially modulates the transcription of p53 target genes.
  • Mts1 enhances p53-dependent apoptosis.

Conclusions:

  • Mts1 physically and functionally interacts with p53.
  • Mts1 acts as a negative regulator of p53 activity.
  • Mts1-mediated inhibition of p53 may contribute to tumor progression and a more aggressive phenotype.

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