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Peptide display in functional genomics
1SELEXIS SA, CBUE-LBTM, EPFL, Via della Ricerca Scientifica, Lausanne, 1015, Switzerland. igor.fisch@iba.unil.ch
Combinatorial Chemistry & High Throughput Screening
|April 3, 2001
Summary
The human genome project fuels functional genomics and proteomics research. High-throughput screening methods accelerate drug discovery by identifying novel protein targets and small molecule ligands, including peptides.
Area of Science:
- Genomics and Proteomics
- Biotechnology
Background:
- The human genome project has spurred advancements in functional genomics, structural genomics, and proteomics.
- These fields aim to identify all proteins within a single cell at specific lifecycle stages.
- High-throughput screening (HTS) methods have driven bioscience expansion and attracted biotechnology investment.
Purpose of the Study:
- To accelerate the validation of novel drug targets using HTS methods.
- To identify small, target-specific molecular ligands.
- To explore molecular diversity through combinatorial methods.
Main Methods:
- Utilizing high-throughput screening (HTS) for rapid target validation.
- Employing combinatorial chemistry for synthesizing and screening molecular libraries.
- Applying recombinant DNA techniques for selecting displayed molecular repertoires.
Main Results:
- Development of new methods for rapid synthesis and screening of small molecules.
- Emergence of techniques for identifying low molecular weight peptides.
- Facilitation of faster drug development through rapid target validation.
Conclusions:
- HTS methods are crucial for accelerating drug discovery and development.
- Combinatorial methods and recombinant DNA techniques are key to exploring molecular diversity.
- Low molecular weight peptides are increasingly important as molecular tools and for generating lead compounds.