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Peptide display in functional genomics.

¹SELEXIS SA, CBUE-LBTM, EPFL, Via della Ricerca Scientifica, Lausanne, 1015, Switzerland. igor.fisch@iba.unil.ch

Combinatorial Chemistry & High Throughput Screening

|April 3, 2001

Summary

The human genome project fuels functional genomics and proteomics research. High-throughput screening methods accelerate drug discovery by identifying novel protein targets and small molecule ligands, including peptides.

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Area of Science:

Genomics and Proteomics
Biotechnology

Background:

The human genome project has spurred advancements in functional genomics, structural genomics, and proteomics.
These fields aim to identify all proteins within a single cell at specific lifecycle stages.
High-throughput screening (HTS) methods have driven bioscience expansion and attracted biotechnology investment.

Purpose of the Study:

To accelerate the validation of novel drug targets using HTS methods.
To identify small, target-specific molecular ligands.
To explore molecular diversity through combinatorial methods.

Main Methods:

Utilizing high-throughput screening (HTS) for rapid target validation.
Employing combinatorial chemistry for synthesizing and screening molecular libraries.
Applying recombinant DNA techniques for selecting displayed molecular repertoires.

Main Results:

Development of new methods for rapid synthesis and screening of small molecules.
Emergence of techniques for identifying low molecular weight peptides.
Facilitation of faster drug development through rapid target validation.

Conclusions:

HTS methods are crucial for accelerating drug discovery and development.
Combinatorial methods and recombinant DNA techniques are key to exploring molecular diversity.
Low molecular weight peptides are increasingly important as molecular tools and for generating lead compounds.

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