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Dehydrogenases and transaminases in asymmetric synthesis
1Department of Chemistry, University of Florida, Gainesville 32611, USA. jds2@chem.ufl.edu
Current Opinion in Chemical Biology
|April 3, 2001
Abstract:
Improved stereoselectivity in dehydrogenase-mediated reductions has been achieved by rationally designed gene overexpression and knockouts in Saccharomyces cerevisiae cells and by isolating and characterizing novel dehydrogenases from other organisms. Transaminases have been used to prepare unnatural amines and amino acids in good yields, particularly when the equilibria are shifted by selective product removal.