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Evidence for tumor necrosis factor receptors (TNFRs) in human MRC5 fibroblast cells
D Derouich-Guergour1, H Pelloux, D Aldebert
1Laboratoire Interactions Cellulaires Parasite-Hôte, EA.UJF 2940, CNRS-ER 2014, Université Joseph-Fourier, Grenoble-I, Domaine de la Merci, 38706 La Tronche Cedex, France. Dorra.Derouich-Guergour@ujf-grenoble.fr
Abstract:
Previous reports have shown that tumor necrosis factor alpha (TNF-alpha) controls the functions of fibroblasts cells, such as proliferation, cell migration and secretion of factors. The signaling for the biological effects of this and other cytokines is usually exerted through cell surface receptors. In this study, we demonstrated the presence of a surface and soluble TNF receptor in MRC5 fibroblasts. The presence of TNFRI and TNFRII mRNA was demonstrated by reverse-transcriptase-PCR (RT-PCR). Both surface TNFRI and TNFRII are expressed and the number of both membrane receptors is 9,251 sites per cell. Using TNF receptor specific antibodies and flow cytometry, we showed that MRC5 cells express mainly TNFRI.
Insights
Fibroblast cells express tumor necrosis factor receptors (TNFRs) on their surface. MRC5 fibroblasts primarily utilize tumor necrosis factor receptor I (TNFRI) for signaling, impacting cell functions.
Area of Science:
- Cell Biology
- Immunology
- Molecular Biology
Background:
- Tumor necrosis factor alpha (TNF-alpha) influences fibroblast functions like proliferation and migration.
- Cytokine signaling typically occurs via cell surface receptors.
Purpose of the Study:
- To investigate the presence and type of TNF receptors on MRC5 fibroblasts.
- To determine the expression levels and primary receptor subtype utilized by these cells.
Main Methods:
- Reverse-transcriptase PCR (RT-PCR) to detect TNFRI and TNFRII mRNA.
- Flow cytometry with specific antibodies to identify surface receptor expression.
- Quantification of receptor sites per cell.
Main Results:
- MRC5 fibroblasts express both TNFRI and TNFRII mRNA.
- Both TNFRI and TNFRII are present on the cell surface, with approximately 9,251 receptor sites per cell.
- Flow cytometry confirmed that MRC5 cells predominantly express TNFRI.
Conclusions:
- MRC5 fibroblasts possess functional TNF receptors on their surface.
- The primary TNF receptor subtype expressed by MRC5 cells is TNFRI, suggesting its key role in mediating TNF-alpha effects on these cells.