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Long-range (1)H-(15)N heteronuclear shift correlation at natural abundance: a tool to study benzothiazole
P Besse1, B Combourieu, G Boyse
1Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 du CNRS, Université Blaise Pascal, 63177 Aubière Cedex, France.
Abstract:
The biodegradation of benzothiazole and 2-hydroxybenzothiazole by two strains of Rhodococcus was monitored by reversed phase high-pressure liquid chromatography and by (1)H nuclear magnetic resonance (NMR). Both xenobiotics were biotransformed into a hydroxylated derivative of 2-hydroxybenzothiazole by these two strains. The chemical structure of this metabolite was determined by a new NMR methodology: long-range (1)H-(15)N heteronuclear shift correlation without any previous (15)N enrichment of the compound. This powerful NMR tool allowed us to assign the metabolite structure to 2,6-dihydroxybenzothiazole.
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