Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Homogeneous assays for single-nucleotide polymorphism typing using AlphaScreen.

L Beaudet1, J Bédard, B Breton

  • 1BioSignal Packard Inc., Montréal, Québec, Canada H3J 1R4. lbeaudet@biosignal.com

Genome Research
|April 3, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rare susceptibility variants for bipolar disorder suggest a role for G protein-coupled receptors.

Molecular psychiatry·2017
Same author

Effects of simulated feeding by snow geese on Scirpus americanus rhizomes.

Oecologia·2017
Same author

Hormonal changes during meiotic maturation and ovulation in the brook trout (Salvelinus fontinalis).

Fish physiology and biochemistry·2013
Same author

Effects of (D-Ala(6), Pro (9)N ethylamide) - LHRH on plasma levels of gonadotropin, 17α,20β-dihydroxy-4-pregnen-3-one and testosterone in male goldeye (hiodon alosoides Rafineque).

Fish physiology and biochemistry·2013
Same author

Gonadotropic hormone (GtH) receptors in the testis of the troutSalmo gairdneri: in vitro studies.

Fish physiology and biochemistry·2013
Same author

Studies on the mode of action of neuropeptide Y (NPY) on maturational gonadotropin (GtH) secretion from perifused rainbow trout pituitary glands.

Fish physiology and biochemistry·2013
Same journal

High-accuracy SNV calling for bacterial isolates using deep learning with AccuSNV.

Genome research·2026
Same journal

Complete sequencing of medaka genomes reveals the architecture of centromeric satellites, giant mobile elements, and sex chromosomes.

Genome research·2026
Same journal

Convergence and conflict among telomere specialized transposons across 60 million years of Drosophilid evolution.

Genome research·2026
Same journal

A unified analysis of cell type- and trajectory-associated pathways in single-cell data using Phoenix.

Genome research·2026
Same journal

Resf1 is required for proper placental development and configuration of trophoblast cell-specific heterochromatin.

Genome research·2026
Same journal

Telomere-driven replicative crisis is driven by large-scale changes in genomic architecture.

Genome research·2026
See all related articles

AlphaScreen technology enables high-throughput, homogeneous proximity assays for nucleic acid detection and single-nucleotide polymorphism (SNP) genotyping. This robust method achieves high accuracy with minimal genomic DNA, simplifying complex genetic analyses.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • AlphaScreen is a homogeneous proximity assay technology.
  • It generates signal upon bead proximity, useful for detecting nucleic acids.
  • The technology enables sensitive detection of single-stranded DNA targets.

Purpose of the Study:

  • To develop and validate AlphaScreen-based platforms for high-throughput single-nucleotide polymorphism (SNP) genotyping.
  • To combine AlphaScreen with allele-specific amplification (ASA) and allele-specific hybridization (ASH) for genotyping applications.
  • To assess the robustness, accuracy, and scalability of these homogeneous genotyping assays.

Main Methods:

  • Utilized AlphaScreen technology with bridging probes for nucleic acid detection.

Related Experiment Videos

  • Developed two homogeneous genotyping platforms integrating AlphaScreen with ASA and ASH.
  • Validated the assays using 12 SNPs (ASA) and 7 SNPs (ASH) across over 580 samples.
  • Performed genotyping in 96- and 384-well formats with low-volume reactions (2 microL).

    • Main Results:

    • Achieved accurate SNP genotyping with less than 2 ng of genomic DNA per genotype.
    • Demonstrated high genotyping accuracy exceeding 99% across all validated SNPs.
    • Showcased the simplicity of the assays, requiring no post-PCR manipulations.
    • Successfully scaled genotyping to 96- and 384-well formats, reducing reagent and DNA requirements.

    Conclusions:

    • AlphaScreen technology is effectively adapted for robust, high-throughput SNP genotyping.
    • The developed ASA and ASH assays offer a simple, accurate, and scalable solution for genetic analysis.
    • This approach significantly reduces the need for reagents and genomic DNA in large-scale genotyping studies.